1OK2

Decay accelerating factor (CD55): the structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	47.580, 55.620, 61.530
Unit cell angles	86.56, 84.44, 64.22

Refinement procedure

Resolution	19.000 - 2.500
R-free	0.27400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h03
RMSD bond length	0.010
RMSD bond angle	19.850 *
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.070	0.260
Number of reflections	18310
<I/σ(I)>	15.4	11.9
Completeness [%]	99.0	98
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 10% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))