1OK1
Decay accelerating factor (cd55) : the structure of an intact human complement regulator.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 47.034, 55.133, 62.001 |
Unit cell angles | 86.85, 84.92, 65.03 |
Refinement procedure
Resolution | 16.000 - 2.600 |
R-free | 0.25900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h03 |
RMSD bond length | 0.006 |
RMSD bond angle | 19.780 * |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | TNT (5F) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.174 |
Number of reflections | 16177 | |
<I/σ(I)> | 5.6 | 3.9 |
Completeness [%] | 93.5 | 90 |
Redundancy | 7.6 | 7.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.6 | 4 * | 0.2 M AMMONIUM SULPHATE, 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 10% GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
2 | 1 | reservoir | MPEG5000 | 20 (%(w/v)) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
4 | 1 | reservoir | glycerol | 10 (%(w/v)) |