1OJW

Decay accelerating factor (CD55): the structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-15
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	53.099, 51.892, 64.125
Unit cell angles	78.39, 83.66, 63.22

Refinement procedure

Resolution	25.000 * - 2.800 *
R-free	0.28800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h03
RMSD bond length	0.005
RMSD bond angle	0.600 *
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500 *	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.135	0.388
Number of reflections	14573
<I/σ(I)>	15.37	3.43
Completeness [%]	99.0	98.4
Redundancy	6.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6 10% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))