1OJV

Decay accelerating factor (CD55): the structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-15
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	46.670, 55.080, 61.650
Unit cell angles	86.47, 84.37, 64.91

Refinement procedure

Resolution	16.000 - 2.300
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h03
RMSD bond length	0.008 *
RMSD bond angle	0.800 *
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000 *	2.420
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.084 *	0.245 *
Number of reflections	29985 *
<I/σ(I)>	6.1	4
Completeness [%]	93.8 *	90.6 *
Redundancy	8.9 *	3.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE, 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 16% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))