1OJR
L-rhamnulose-1-phosphate aldolase from Escherichia coli (mutant E192A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 108.024, 108.024, 57.166 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.500 * - 1.350 |
R-factor | 0.112 |
Rwork | 0.111 |
R-free | 0.14200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gt7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.250 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.500 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.052 | 0.380 |
Number of reflections | 71387 | 7660 * |
<I/σ(I)> | 8.7 | 1.7 |
Completeness [%] | 96.0 | 95 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3 * | HANGING DROP WITH 5 MG/ML PROTEIN AND 18% (V/V) DIOXANE. RESERVOIR WITH 35% (V/V) DIOXANE. HAMPTON CRYSTAL SCREEN-2 NO.4, pH 4.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | potassium phosphate | 20 (mM) | pH3.0 |
2 | 1 | reservoir | dioxane | 35 (%(v/v)) | |
3 | 1 | drop | enzyme | 10 (mg/ml) |