1OI6
Structure determination of the TMP-complex of EvaD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.500, 72.571, 57.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.100 - 1.400 |
| R-factor | 0.13 |
| Rwork | 0.129 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofn |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.803 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.074 | 0.450 |
| Number of reflections | 80059 | |
| <I/σ(I)> | 6.3 | 1.5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 25% PEG4K, 100MM MGCL2, 100MM TRIS-HCL, PH8.0, SITTING DROP, pH 8.00 |
