1OHW
4-AMINOBUTYRATE-AMINOTRANSFERASE inactivated by gamma-vinyl GABA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.660, 226.712, 71.366 |
| Unit cell angles | 90.00, 108.77, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22400 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.160 |
| Number of reflections | 88767 | |
| <I/σ(I)> | 6.6779 | 4.14 |
| Completeness [%] | 96.1 | 96.7 |
| Redundancy | 2.2 * | 2.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.4 * | pH 5.70 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-14 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 40 (mM) | pH5.4 |
| 3 | 1 | reservoir | PEG4000 | 16-18 (%) | |
| 4 | 1 | reservoir | glycerol | 0-10 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.0 |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
