1OGV

Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-05
Detector	ADSC CCD
Spacegroup name	P 42 21 2
Unit cell lengths	99.973, 99.973, 237.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.300 * - 2.350
R-factor	0.214
Rwork	0.214
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aij
RMSD bond length	0.007
RMSD bond angle	1.230 *
Data reduction software	MOSFLM (V. 6.2)
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.300	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.115	0.668
Number of reflections	51199 *
<I/σ(I)>	12	2.8
Completeness [%]	96.3	97.6
Redundancy	7.2	7.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	solid cubic phase *	7.5		60% MONOOLEIN/40% 25 MG/ML PROT. +(4:1)18% JEFFAMINE M-600, 1M HEPES PH7.5, 0.7M AMM.SULF., pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2.0 (mg/ml)
2	1	2	monoolein	12 (%(w/v))
3	1	2	Jeffamine M-600	14.4 (%(w/v))
4	1	2	HEPES	0.8 (M)	pH7.5
5	1	2	ammonium sulfate	0.56 (M)
6	1	2	LDAO	0.008 (%)