1OFW

Three dimensional structure of the oxidized form of nine heme cytochrome c at PH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	60.300, 105.950, 80.370
Unit cell angles	90.00, 103.18, 90.00

Refinement procedure

Resolution	25.000 - 1.500
R-factor	0.1758
R-free	0.19300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	19hc
RMSD bond length	0.010
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.036	0.269
Total number of observations	330192 *
Number of reflections	153684
<I/σ(I)>	19	3
Completeness [%]	98.2	96.8
Redundancy	2.14	2.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		Coelho, A.V., (1996) Protein Sci., 5, 1189. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%(w/v))
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir		0.2 (M)