1OF6
crystal structure of the tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from saccharomyces cerevisiae complexed with tyrosine and manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.129, 94.696, 104.843 |
| Unit cell angles | 64.71, 85.51, 75.61 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| Rwork | 0.195 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MOLECULE A OF PDB-CODE 1HFB |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.996 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.067 | 0.280 |
| Number of reflections | 407127 | |
| <I/σ(I)> | 10.1 | 2.1 |
| Completeness [%] | 96.5 | 89.1 |
| Redundancy | 2.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.00 |
