1OE8
28kDa glutathione S-transferase from Schistosoma haematobium (glutathione saturated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.142, 77.705, 53.195 |
| Unit cell angles | 90.00, 93.40, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.650 |
| Rwork | 0.229 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gtb |
| RMSD bond length | 0.023 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 51735 | |
| <I/σ(I)> | 40.9 | 2.7 |
| Completeness [%] | 99.2 | 95 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | PROTEIN (~60MG/ML) WITH 500UM EXCESS OF GSH WAS CRYSTALLIZED IN HANGING DROPS USING A WELL SOLUTION OF 2.1M AMMONIUM SULFATE, 100MM TRIS, PH7.2, 5MM BETA-MERCAPTOETHANOL., pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH7.2 |
| 3 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 4 | 1 | drop | protein | 66 (mg/ml) |
