1ODO
1.85 A structure of CYP154A1 from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.510, 103.708, 104.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.210 - 1.850 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.468 * |
| Number of reflections | 44967 | |
| <I/σ(I)> | 17.6 | 3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.0 * | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 22 * | 0.7 M SODIUM CITRATE 0.1 M HEPES, PH=7.5, 0 5 MM 4-PHENYLIMIDAZOLE, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.3 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 0.7 (M) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | 4-phenylimidazole | 5 (mM) |
