1ODJ
PURINE NUCLEOSIDE PHOSPHORYLASE FROM THERMUS THERMOPHILUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-15 |
Detector | RIGAKU IMAGE PLATE, RAXIS-VII |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 132.757, 132.757, 172.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.930 - 2.400 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | THE REFINED STRUCTURE OF PURINE NUCLEOSIDE PEPTIDASE FROM THERMUS THERMOPHILUS WAS USED AS STARTING MODEL FOR REFINEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.118 | 0.332 |
Number of reflections | 58580 | |
<I/σ(I)> | 16.9 | 3.9 |
Completeness [%] | 96.9 | 92.7 |
Redundancy | 4.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.3 | 291 | MICROBATCH METHOD UNDER OIL WAS USED. 15.1 MG/ML OF PROTEIN SOLUTION CONTAINING 0.02M DTT WAS MIXED WITH 1.65M SODIUM ACETATE AND 0.1M MES PH 6.3. THE CRYSTALLIZATION TEMPERATURE WAS 291 K. PARATONE-N OIL MIXED WITH 10% W/W OF GLYCEROL WAS USED FOR CRYOPROTECTION LIGAND BOUND CRYSTALS WERE OBTAINED BY 7 H SOAKING OF NATIVE CRYSTALS IN SOLUTION CONTAINING 1.2M SODIUM ACETATE PH 6.3, 20MM AMMONIUM SULFATE, 2MM MAGNESIUM SULFATE AND 5MM GUANOSINE |