1OC4

Lactate dehydrogenase from Plasmodium berghei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	103.586, 71.528, 94.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.300
R-factor	0.222
Rwork	0.219
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ldg
RMSD bond length	0.011
RMSD bond angle	1.428 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.087	0.281
Total number of observations	173810 *
Number of reflections	31653
<I/σ(I)>	18.11	4.01
Completeness [%]	98.8	98
Redundancy	5.49	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		32% PEG 1500, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	NADH	2.5 (mM)
2	1	reservoir	PEG1500	32-34 (%)
3	1	reservoir	MPD	65 (%)