1OC4
Lactate dehydrogenase from Plasmodium berghei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.586, 71.528, 94.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.300 |
R-factor | 0.222 |
Rwork | 0.219 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ldg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.428 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.087 | 0.281 |
Total number of observations | 173810 * | |
Number of reflections | 31653 | |
<I/σ(I)> | 18.11 | 4.01 |
Completeness [%] | 98.8 | 98 |
Redundancy | 5.49 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 32% PEG 1500, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | NADH | 2.5 (mM) | |
2 | 1 | reservoir | PEG1500 | 32-34 (%) | |
3 | 1 | reservoir | MPD | 65 (%) |