1OBG
SAICAR-synthase complexed with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.770, 74.190, 76.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.800 - 2.050 |
R-factor | 0.168 |
Rwork | 0.166 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a48 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.820 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.070 | 0.190 |
Number of reflections | 25206 | |
<I/σ(I)> | 17 | 3.6 |
Completeness [%] | 98.5 | 98.5 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 40MM ASP. ASID, 10MM MGCL2, 25MM ATP, 2.26 M (NH4)2(SO4), 5, pH 4.50 |