1OBC
LEUCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH A POST-TRANSFER EDITING SUBSTRATE ANALOGUE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-09 |
| Detector | CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.896, 154.810, 175.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.243 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.088 | 0.350 |
| Number of reflections | 77419 | |
| <I/σ(I)> | 6.3 | 2 |
| Completeness [%] | 96.2 | 84.2 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | used microseeding, Yaremchuk, A., (2000) Acta Crystallogr., 56, 667. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris propane | 50 (mM) | pH6.0-6.5 |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | ammonium sulfate | 15 (%sat) | |
| 7 | 1 | reservoir | ammonium sulfate | 30 (%) |
