1OAB
CRYSTAL STRUCTURE OF THE TYROSINE REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH PHOSPHOENOLPYRUVATE AND MANGANESE(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.636, 50.502, 64.930 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 19.900 * - 1.900 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hfb |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 * | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.105 * | 0.380 |
| Total number of observations | 178093 * | |
| Number of reflections | 48773 * | |
| <I/σ(I)> | 8.34 | 2.9 |
| Completeness [%] | 99.3 | 96.9 * |
| Redundancy | 3.63 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 4 * | TRIS PH 7.5-9.0 10 MM, 20% PEG3400, 5% GLYCEROL, 4 EQUIV. PEP, 13-17MG/ML DAHPS |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13-18 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5-9 |
| 3 | 1 | reservoir | PEG3400 | 9-17 (%(w/v)) | |
| 4 | 1 | reservoir | glycerol | 5 (%(v/v)) | |
| 5 | 1 | reservoir | Tris | 10 (mM) | pH7.5-9 |
