1O9J
The X-ray crystal structure of eta-crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.869, 136.407, 84.988 |
Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
Resolution | 41.520 - 2.400 |
R-factor | 0.184 |
Rwork | 0.180 |
R-free | 0.26200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bxs |
RMSD bond length | 0.011 |
RMSD bond angle | 1.280 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.068 | 0.174 |
Total number of observations | 159192 * | |
Number of reflections | 67543 | 8412 * |
<I/σ(I)> | 12.2 | 7.2 |
Completeness [%] | 96.5 | 94.7 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | 25 MM BIS-TRIS-PROPANE PH 7.5, APPROXIMATELY 14% PEG 6,000. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Bis-Tris-HCl | 25 (mM) | |
2 | 1 | drop | EDTA | 1 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | pH6.5 |
4 | 1 | drop | protein | 8 (mg/ml) | |
5 | 1 | reservoir | Bis-Tris propane | 25 (mM) | pH7.5 |
6 | 1 | reservoir | PEG6000 | 14 (%) |