1O7J
Atomic resolution structure of Erwinia chrysanthemi L-asparaginase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-10 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.380, 90.350, 127.590 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 25.000 * - 1.000 |
R-factor | 0.1098 |
R-free | 0.12830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jsl |
RMSD bond length | 0.015 |
RMSD bond angle | 0.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.040 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.050 | 0.305 |
Total number of observations | 1780326 * | |
Number of reflections | 559626 | |
<I/σ(I)> | 24.2 | 3 |
Completeness [%] | 87.6 | 75.1 |
Redundancy | 3.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | Miller, M., (1993) FEBS Lett., 328, 275. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 50 (%) | pH8-9 |
2 | 1 | reservoir | CHES | 0.1 (M) | |
3 | 1 | reservoir | PEG400 | 2 (%(w/v)) |