1O5Z
Crystal structure of Folylpolyglutamate synthase (TM0166) from Thermotoga maritima at 2.10 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-25 |
| Detector | ADSC |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.751, 84.751, 280.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.190 - 2.100 |
| R-factor | 0.1842 |
| Rwork | 0.181 |
| R-free | 0.24421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fgs |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.690 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 33188 | |
| <I/σ(I)> | 22.2 | 2.57 |
| Completeness [%] | 100.0 | 61.61 |
| Redundancy | 5.57 | 3.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.7 | 277 | 0.2 M Potassium Sulfate, 20% w/v/ PEG 3350, pH6.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
