1O5R
Crystal structure of adenosine deaminase complexed with a potent inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-30 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 0.836 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.440, 78.440, 137.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.350 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Phasing software | AMoRE |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.750 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 27278 | |
Completeness [%] | 99.0 | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Ammonium sulphate, PEG400, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |