1O58
Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.80 A resolution
Replaces: 1J6NExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97999, 0.97980, 0.91837 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.570, 84.590, 107.220 |
Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
Resolution | 48.660 - 1.800 |
R-factor | 0.15938 |
Rwork | 0.157 |
R-free | 0.19800 * |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.410 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 * | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.038 * | 0.273 * |
Total number of observations | 400214 * | |
Number of reflections | 97122 | |
<I/σ(I)> | 20.2 | 4.3 |
Completeness [%] | 87.6 | 69.7 * |
Redundancy | 4.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.9 * | 293 | 50% PEG-400, 0.1M CHES pH 9.5, 0.2M NaCl, pH 9.2, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 20 (mM) | pH7.9 |
2 | 1 | reservoir | 150 (mM) | ||
3 | 1 | reservoir | TCEP | 0.25 | |
4 | 1 | drop | protein | 13 (mg/ml) |