Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-24 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 129.310, 74.830, 78.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.000 - 2.600 |
Rwork | 0.218 |
R-free | 0.29700 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 25.954 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.000 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.063 | 0.387 |
Number of reflections | 24126 | |
<I/σ(I)> | 8.8 | 2 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 6.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 295 | 42.5% saturated ammonium sulphate, 100mM Tris ph 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.5 |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | sodium azide | 0.05 (%) | |
5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
6 | 1 | reservoir | ammonium sulfate | 40-45 (%) | |
7 | 1 | reservoir | sodium azide | 0.1 (%) |