1O07
Crystal Structure of the complex between Q120L/Y150E mutant of AmpC and a beta-lactam inhibitor (MXG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.808, 76.298, 98.127 |
| Unit cell angles | 90.00, 116.21, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.710 |
| R-factor | 0.15552 |
| Rwork | 0.154 |
| R-free | 0.19100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Isotropic with individual B factors refined |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.041 | 0.122 |
| Total number of observations | 440555 * | |
| Number of reflections | 83273 | |
| <I/σ(I)> | 32.7 | 10.7 |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 5.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.7 | 295 | Potassium Phosphate buffer, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.9 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 1.0 (M) | pH8.7 |
| 3 | 1 | reservoir | potassium phosphate | 1.7 (M) | pH8.7 |
