1NZC
The high resolution structures of RmlC from Streptococcus suis in complex with dTDP-D-xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2001-10-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.850, 140.866, 53.699 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.800 |
| R-factor | 0.16698 |
| Rwork | 0.164 |
| R-free | 0.21600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nyw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.580 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.790 | 1.810 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.117 * | 0.085 * |
| Total number of observations | 659635 * | |
| Number of reflections | 63995 * | |
| <I/σ(I)> | 3.8 | 8.1 |
| Completeness [%] | 91.8 | 43.3 |
| Redundancy | 5.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 0.1 M Tris, 4 mM NiCl2,10 mM dTDP-D-xylose, 35% PEG 2000, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.2 |
| 3 | 1 | reservoir | 4 (mM) | ||
| 4 | 1 | reservoir | PEG2000 | 35 (%) | |
| 5 | 1 | drop | dTDP-D-xylose | 10 (mM) |
