1NYS
Crystal Structure of Activin A Bound to the ECD of ActRIIB P41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2002-05-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 |
Unit cell lengths | 104.954, 104.954, 46.199 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 3.050 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.29800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bte |
RMSD bond length | 0.011 |
RMSD bond angle | 1.840 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.100 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.067 | 0.435 |
Total number of observations | 44194 * | |
Number of reflections | 9789 | |
<I/σ(I)> | 22.14 | 2.9 |
Completeness [%] | 93.5 | 87 * |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | PEG 4000, sodium chloride, hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-10 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | 150 (mM) | pH7.5 | |
4 | 1 | reservoir | PEG4000 | 27 (%) | |
5 | 1 | reservoir | 250 (mM) | ||
6 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |