1NY6

Crystal structure of sigm54 activator (AAA+ ATPase) in the active state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	106.788, 108.265, 110.020
Unit cell angles	70.25, 85.90, 73.27

Refinement procedure

Resolution	20.000 * - 3.100
R-factor	0.266
Rwork	0.266
R-free	0.32900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	22.600 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.020	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.061	0.421 *
Number of reflections	86929
<I/σ(I)>	7.8
Completeness [%]	98.0	97.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	diammonium tartrate, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	diammonium tartrate	50 (mM)	pH6.5
3	1	reservoir	PEG3350	8 (%(v/v))