1NY6
Crystal structure of sigm54 activator (AAA+ ATPase) in the active state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 106.788, 108.265, 110.020 |
Unit cell angles | 70.25, 85.90, 73.27 |
Refinement procedure
Resolution | 20.000 * - 3.100 |
R-factor | 0.266 |
Rwork | 0.266 |
R-free | 0.32900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 22.600 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.020 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.061 | 0.421 * |
Number of reflections | 86929 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 98.0 | 97.1 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | diammonium tartrate, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | diammonium tartrate | 50 (mM) | pH6.5 |
3 | 1 | reservoir | PEG3350 | 8 (%(v/v)) |