1NY5
Crystal structure of sigm54 activator (AAA+ ATPase) in the inactive state
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.765, 94.765, 195.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.600 * - 2.400 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.415 * |
| Number of reflections | 40276 | |
| <I/σ(I)> | 35.3 | 3 |
| Completeness [%] | 99.4 | 83.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium/potasium phosphate, citric acid,imidazole,methanol,glycerol,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 0.6-0.7 (M) | ||
| 3 | 1 | reservoir | citric acid | 100 (mM) | pH6.5 |
| 4 | 1 | reservoir | imidazole | 10 (mM) | |
| 5 | 1 | reservoir | methanol | 3 (%(v/v)) |
