1NW6
Structure of the beta class N6-adenine DNA methyltransferase RsrI bound to sinefungin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.428, 130.070, 67.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 * - 1.940 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eg2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.038 * | 0.167 * |
Total number of observations | 78952 * | |
Number of reflections | 23268 | 2292 * |
<I/σ(I)> | 22.5 | 6.5 |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.4 | 298 | lithium sulfate, HEPES, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.0 (mg/ml) | |
2 | 1 | reservoir | sinefungin | 1-20 (mM) | |
3 | 1 | reservoir | HEPES | 100 (mM) | pH7.4 |
4 | 1 | reservoir | 1.5 (M) |