1NUV
The Leadzyme Ribozyme Bound to Mg(H2O)6(II) and Sr(II) at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 60.600, 60.600, 133.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.900 * - 1.800* |
| Rwork | 0.210 |
| R-free | 0.22000 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | NDB ENTRY UR0001 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 * | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.035 * | 0.164 * |
| Total number of observations | 228937 * | |
| Number of reflections | 24885 | |
| <I/σ(I)> | 22.5 | 4.6 |
| Completeness [%] | 94.1 | |
| Redundancy | 2.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | MPD, MG-ACETATE, CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MG-ACETATE, | ||
| 3 | 1 | 1 | CACODYLATE | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 3 | SrCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 21-24 (%(v/v)) | ||
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | spermine-HCl | 1 (mM) | |
| 4 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.0 |
