1NUN

Crystal Structure Analysis of the FGF10-FGFR2b Complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	90
Detector technology	CCD
Collection date	2001-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97880, 0.97933, 0.97169
Spacegroup name	P 64 2 2
Unit cell lengths	113.930, 113.930, 164.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.900
R-factor	0.232
Rwork	0.239
R-free	0.28800
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.140
High resolution limit [Å]	2.900	2.900
Rmerge	0.064 *	0.279 *
Total number of observations	279164 *
Number of reflections	14520 *
Completeness [%]	99.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	PEG 400, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	3 (%)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
4	1	drop	protein	7 (mg/ml)
5	1	drop	HEPES	25 (mM)	pH7.5
6	1	drop		150 (mM)