1NUN
Crystal Structure Analysis of the FGF10-FGFR2b Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97880, 0.97933, 0.97169 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 113.930, 113.930, 164.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.900 |
| R-factor | 0.232 |
| Rwork | 0.239 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.140 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.064 * | 0.279 * |
| Total number of observations | 279164 * | |
| Number of reflections | 14520 * | |
| Completeness [%] | 99.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | PEG 400, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 3 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 4 | 1 | drop | protein | 7 (mg/ml) | |
| 5 | 1 | drop | HEPES | 25 (mM) | pH7.5 |
| 6 | 1 | drop | 150 (mM) |
