1NU2
Crystal structure of the murine Disabled-1 (Dab1) PTB domain-ApoER2 peptide-PI-4,5P2 ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-29 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.270, 45.984, 89.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
R-factor | 0.2384 |
Rwork | 0.204 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ntv |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.080 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.960 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.047 | 0.170 |
Total number of observations | 34852 * | |
Number of reflections | 12179 | |
Completeness [%] | 98.0 | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 298 | HEPES, PEG8000, ethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-25 (mg/ml) | |
2 | 1 | drop | Tris | 50 (mM) | pH6.8 |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
6 | 1 | drop | PEG8000 | 34-36 (%) | |
7 | 1 | drop | ethanol | 5 (%) | |
8 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
9 | 1 | reservoir | PEG8000 | 34-36 (%) |