1NT3
HUMAN NEUROTROPHIN-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Collection date | 1996-12 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 37.434, 52.067, 64.795 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NT3 PROTOMER OF 1BND |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.506 |
| Number of reflections | 6760 | |
| <I/σ(I)> | 7.1 | 1.61 |
| Completeness [%] | 86.1 | 85.2 |
| Redundancy | 4.4 | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 18 * | 200 MM AMMONIUM SULFATE, 32% PEG 400, 100 MM MES AT PH 5.6, pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | ||
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | reservoir | MES | 100 (mM) |
