1NQ2
Two RTH Mutants with Impaired Hormone Binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.954, 68.954, 131.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| Rwork | 0.227 |
| R-free | 0.25900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THYROID HORMONE RECEPTOR BETA WITH TRIAC BOUND (unpublished) |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.610 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.055 * |
| Total number of observations | 54104 * |
| Number of reflections | 13799 * |
| <I/σ(I)> | 105.6 |
| Completeness [%] | 93.3 * |
| Redundancy | 25.122 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 277 | 700 mM sodium acetate, 200mM sodium succinate 100mM sodium cacodylate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | pH8.0 |
| 2 | 1 | drop | dithiothreitol | 3 (mM) | |
| 3 | 1 | drop | protein | 11.5 (mg/ml) | |
| 4 | 1 | reservoir | sodium acetate | 700 (mM) | |
| 5 | 1 | sodium succinate | 200 (mM) | ||
| 6 | 1 | sodium cacodylate | 100 (mM) | pH7.2 |
