1NQ2

Two RTH Mutants with Impaired Hormone Binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.08
Spacegroup name	P 31 2 1
Unit cell lengths	68.954, 68.954, 131.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
Rwork	0.227
R-free	0.25900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THYROID HORMONE RECEPTOR BETA WITH TRIAC BOUND (unpublished)
RMSD bond length	0.008 *
RMSD bond angle	1.610 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.400
Rmerge	0.055 *
Total number of observations	54104 *
Number of reflections	13799 *
<I/σ(I)>	105.6
Completeness [%]	93.3 *
Redundancy	25.122

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	277	700 mM sodium acetate, 200mM sodium succinate 100mM sodium cacodylate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)	pH8.0
2	1	drop	dithiothreitol	3 (mM)
3	1	drop	protein	11.5 (mg/ml)
4	1	reservoir	sodium acetate	700 (mM)
5	1		sodium succinate	200 (mM)
6	1		sodium cacodylate	100 (mM)	pH7.2