1NPM
NEUROPSIN, A SERINE PROTEASE EXPRESSED IN THE LIMBIC SYSTEM OF MOUSE BRAIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11 |
Spacegroup name | P 1 |
Unit cell lengths | 38.400, 55.160, 65.370 |
Unit cell angles | 95.38, 89.98, 110.46 |
Refinement procedure
Resolution | 100.000 - 2.100 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4PTP |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.535 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.240 | |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.198 |
Number of reflections | 24944 | |
<I/σ(I)> | 8.5 | 3.1 |
Completeness [%] | 90.7 * | 74.5 |
Redundancy | 2.5 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 24.5 (mg/ml) | |
2 | 1 | reservoir | HEPES | 5 (mM) | |
3 | 1 | reservoir | 100 (mM) |