1NPL

MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM DAFFODIL (NARCISSUS PSEUDONARCISSUS) BULBS IN COMPLEX WITH MANNOSE-ALPHA1,3-MANNOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-09-19
Detector	MARRESEARCH
Spacegroup name	C 2 2 2
Unit cell lengths	73.300, 101.200, 37.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.187 *
Rwork	0.187
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	0.037
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.067	0.273
Total number of observations	27588 *
Number of reflections	9490	1322 *
<I/σ(I)>	7.26	2.2
Completeness [%]	98.6	98.6 *
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	17 *	VAPOUR DIFFUSION, SITTING DROP, 10 MG/ML IN PBS CONTAINING UP TO 20 MM MANNOBIOSE, EQUILIBRATED AGAINST 40 - 60% AMMONIUM SULPHATE. 17 DEG. C, 4 - 6 DAYS, pH 6.5, vapor diffusion - sitting drop, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	phosphate
3	1	drop	alpha-1,3 mannobiose	20 (mM)
4	1	reservoir	ammonium sulfate	40-60 (%)	pH6.5