1NPL
MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM DAFFODIL (NARCISSUS PSEUDONARCISSUS) BULBS IN COMPLEX WITH MANNOSE-ALPHA1,3-MANNOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 73.300, 101.200, 37.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.187 * |
| Rwork | 0.187 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.273 |
| Total number of observations | 27588 * | |
| Number of reflections | 9490 | 1322 * |
| <I/σ(I)> | 7.26 | 2.2 |
| Completeness [%] | 98.6 | 98.6 * |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 17 * | VAPOUR DIFFUSION, SITTING DROP, 10 MG/ML IN PBS CONTAINING UP TO 20 MM MANNOBIOSE, EQUILIBRATED AGAINST 40 - 60% AMMONIUM SULPHATE. 17 DEG. C, 4 - 6 DAYS, pH 6.5, vapor diffusion - sitting drop, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | phosphate | ||
| 3 | 1 | drop | alpha-1,3 mannobiose | 20 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 40-60 (%) | pH6.5 |
