1NPB

Crystal structure of the fosfomycin resistance protein from transposon Tn2921

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	113
Detector technology	CCD
Collection date	2002-08-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9797
Spacegroup name	I 4 2 2
Unit cell lengths	208.597, 208.597, 136.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.500
R-factor	0.18457
Rwork	0.183
R-free	0.23040 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lqo
RMSD bond length	0.016
RMSD bond angle	1.672
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000 *	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.082 *	0.442 *
Number of reflections	51262
<I/σ(I)>	20.5	2.7
Completeness [%]	98.9	90.3
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	22 *	1.6M ammonium sulfate, 100 mM sodium citrate, 5% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop		0.6 (mM)
3	1	drop	fosfomycin	0.6 (mM)
4	1	reservoir	ammonium sulfate	1.6 (M)
5	1	reservoir	sodium citrate	100 (mM)	pH6.5
6	1	reservoir	glycerol	5 (%)