1NPB
Crystal structure of the fosfomycin resistance protein from transposon Tn2921
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-08-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 208.597, 208.597, 136.358 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.500 |
| R-factor | 0.18457 |
| Rwork | 0.183 |
| R-free | 0.23040 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lqo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.672 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 * | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 * | 0.442 * |
| Number of reflections | 51262 | |
| <I/σ(I)> | 20.5 | 2.7 |
| Completeness [%] | 98.9 | 90.3 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 22 * | 1.6M ammonium sulfate, 100 mM sodium citrate, 5% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | 0.6 (mM) | ||
| 3 | 1 | drop | fosfomycin | 0.6 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.5 |
| 6 | 1 | reservoir | glycerol | 5 (%) |
