1NP3
Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-15 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 184.377, 184.377, 184.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.23600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.160 * |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.010 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | |
| Number of reflections | 135868 | |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 297 * | Eom, S.J., (2002) Acta Crystallogr., D58, 2145. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 22.2 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
| 3 | 1 | reservoir | sodium acetate | 1.4 (M) |
