1NOU
Native human lysosomal beta-hexosaminidase isoform B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.465, 112.465, 397.867 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 * - 2.400 |
R-factor | 0.20266 |
Rwork | 0.201 |
R-free | 0.23100 * |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.590 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | |
High resolution limit [Å] | 2.400 | 2.400 * |
Rmerge | 0.090 * | 0.314 * |
Total number of observations | 2772717 * | |
Number of reflections | 58844 | 2894 * |
Completeness [%] | 99.5 * | 99.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Mahuran, D.J., (1980) Can. J. Biochem., 58, 287. * |