1NNP
X-ray structure of the GluR2 ligand-binding core (S1S2J) in complex with (S)-ATPA at 1.9 A resolution. Crystallization without zinc ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.07600 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.728, 121.469, 47.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.190 |
| R-free | 0.21700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GluR2:(S)-ATPA complex (zinc form). |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.348 |
| Number of reflections | 40611 | |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 92.2 | 81.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 6 * | ammonium sulfate, PEG8000, sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | GluR2-S1S2J | 1.4 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.6 |
| 5 | 1 | reservoir | PEG8000 | 18 (%) |
