1NNK
X-ray structure of the GluR2 ligand-binding core (S1S2J) in complex with (S)-ATPA at 1.85 A resolution. Crystallization with zinc ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08350 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.258, 111.229, 46.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.850 |
| Rwork | 0.207 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GluR2:(S)-thio-ATPA complex (Lunn et al. to be published). |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.099 | 0.340 |
| Number of reflections | 23016 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 92.6 | 82.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 6 * | zinc acetate, cacodylate, PEG8000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | GluR2-S1S2J | 1.4 (mg/ml) | |
| 3 | 1 | reservoir | zinc acetate | 0.1 (M) | |
| 4 | 1 | reservoir | cacodylate | 0.1 (M) | pH5.5 |
| 5 | 1 | reservoir | PEG8000 | 20 (%) |
