Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.469, 99.145, 102.261 |
| Unit cell angles | 90.00, 104.14, 90.00 |
Refinement procedure
| Resolution | 24.710 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.29000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.170 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 * | |
| Total number of observations | 224308 * | |
| Number of reflections | 71688 * | |
| <I/σ(I)> | 15 | 1.1 |
| Completeness [%] | 99.0 * | 77 |
| Redundancy | 2.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | PEG 4000, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta4Gal-T1 | 20 (mg/ml) | |
| 2 | 1 | drop | LA | 10 (mg/ml) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 12.5 (%(w/v)) | |
| 6 | 1 | drop | 17 (mM) |
