1NKG
Rhamnogalacturonan lyase from Aspergillus aculeatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.009, 77.009, 170.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 1.500 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.18600 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.108 | 0.292 |
Number of reflections | 82997 | |
<I/σ(I)> | 16.71 | 4.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 277 | 20% PEG 4000, 9% PEG 400, 0.1M (NH4)2SO4, 0.1M sodium acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |