1NI2
Structure of the active FERM domain of Ezrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2000-01-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.901 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.489, 112.802, 66.301 |
Unit cell angles | 90.00, 102.32, 90.00 |
Refinement procedure
Resolution | 26.000 * - 2.300 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.28200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb is 1EF1 |
RMSD bond length | 0.011 * |
RMSD bond angle | 2.200 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 * | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.072 * | 0.256 * |
Total number of observations | 183710 * | |
Number of reflections | 27498 * | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 89.3 | 77.2 * |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 * | 291 | 10% PEG 2K, 10% GLYCEROL, 5% ISOPROPANOL, TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG2000 MME | 10-15 (%(w/v)) | |
2 | 1 | reservoir | glycerol | 15 (%) | |
3 | 1 | reservoir | 2-propanol | 10 (%(w/v)) | |
4 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH8.1 |