1NHJ
Crystal structure of N-terminal 40KD MutL/A100P mutant protein complex with ADPnP and one sodium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.338, 72.640, 190.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.21681 |
Rwork | 0.217 |
R-free | 0.26600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b63 |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.222 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.054 * | 0.490 * |
Number of reflections | 33550 | |
Completeness [%] | 93.6 * | 93.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 20 * | Ban, C., (1999) Cell(Cambridge,Mass.), 97, 85. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
10 | 1 | reservoir | PEG4000 | 18 (%) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | EDTA | 1.0 (mM) | |
5 | 1 | drop | 150 (mM) | ||
6 | 1 | drop | 5 (mM) | ||
7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
8 | 1 | reservoir | 200 (mM) | ||
9 | 1 | reservoir | dithiothreitol | 2 (mM) |