Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97894, 0.97855,0.96334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.171, 99.013, 102.382 |
| Unit cell angles | 90.00, 103.92, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.500 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.30000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 * | |
| Total number of observations | 110000 * | |
| Number of reflections | 37099 * | |
| <I/σ(I)> | 13.6 | 2 |
| Completeness [%] | 98.0 * | 71 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | Sodium citrate, PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta4Gal-T1 | 20 (mg/ml) | |
| 2 | 1 | drop | LA | 10 (mg/ml) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 12.5 (%(w/v)) | |
| 6 | 1 | drop | UDP-Gal | 100 (mM) |
