1NGS

COMPLEX OF TRANSKETOLASE WITH THIAMIN DIPHOSPHATE, CA2+ AND ACCEPTOR SUBSTRATE ERYTHROSE-4-PHOSPHATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	76.500, 113.300, 160.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.206
Rwork	0.206
R-free	0.23900
RMSD bond length	0.011
RMSD bond angle	24.251 *
Data reduction software	MSC
Data scaling software	MSC
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.062
Total number of observations	156468 *
Number of reflections	45990
Completeness [%]	81.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6	4 *	Schneider, G., (1989) J. Biol. Chem., 264, 21619. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	13-16 (%(w/w))
2	1	drop	glycyl-glycine	50 (mM)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir	PEG6000	13-16 (%(w/w))
5	1	reservoir	glycyl-glycine	50 (mM)