1NF1
THE GAP RELATED DOMAIN OF NEUROFIBROMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-26 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.250, 58.300, 74.800 |
| Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.36900 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS ((W.KABSCH)) |
| Data scaling software | XSCALE ((KABSCH)) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 * | 0.300 |
| Total number of observations | 50172 * | |
| Number of reflections | 11665 * | |
| <I/σ(I)> | 11.6 | 3.6 |
| Completeness [%] | 99.8 * | 99.8 |
| Redundancy | 4.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | S. REF. DESCRIBING THE STRUCTURE, pH 8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 18-22 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | drop | Tris-Cl | 30 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | DTE | 3 (mM) | |
| 7 | 1 | drop | protein | 20 (mg/ml) |
