1NDD
STRUCTURE OF NEDD8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.804, 64.972, 48.573 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.600 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.452 |
| Total number of observations | 232354 * | |
| Number of reflections | 36579 | |
| <I/σ(I)> | 20 | 1.9 |
| Completeness [%] | 97.8 | 93.7 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.6 * | 21 * | pH 4.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 40 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | EDTA | 0.4 (mM) | |
| 5 | 1 | drop | dithiothreitol | 0.2 (mM) | |
| 6 | 1 | reservoir | citric acid | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.2 (M) |
