1NCF

A NEW PARADIGM FOR TUMOR NECROSIS FACTOR SIGNALLING

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	69.000, 69.000, 185.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.250
R-factor	0.193
Rwork	0.193
R-free	0.24900
RMSD bond length	0.009
RMSD bond angle	26.020 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.250 *
Rmerge	0.051 *
Number of reflections	21874 *
Completeness [%]	100.0
Redundancy	6.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *	293 *	Rodseth, L.E., (1994) J.Mol.Biol., 239, 332. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.6 (mg/ml)
2	1	drop	MPD	25 (%(v/v))
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	beta-octylglucoside	0.1 (%(w/v))
5	1	drop		100 (mM)
6	1	drop	ammonium acetate	250 (mM)
7	1	reservoir	MPD	54 (%)
8	1	reservoir	Tris-HCl	100 (mM)
9	1	reservoir	ammonium acetate	500 (mM)